High Yield T7 RNA Synthesis Kit Nucleotides

High Yield T7 RNA Synthesis Kit NucleotidesProduct Information The High Yield T7 RNA Synthesis Kit is designed to produce 25 fold more full length RNA transcript per reaction than conventional in vitro transcription reactions. The yield is up to 210g RNA per reaction. Materials provided with the kit: Component 50 rxn kits Storage 5X Reaction Buffer* 200L 20 100mM ATP Solution 100L 20 100mM GTP Solution 100L 20 100mM UTP Solution 100L 20 100mM CTP Solution 100L 20 Enzyme Mix 75L 20 Control

BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5'-GCTCTTCN1/N4-3'

Modifications: N1-Methyl-Pseudo-U

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Magdalena Malgowska

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} 5-Me-DMT-dC(Ac)-CE-Phosphoramidite is a standard DNA phosphoramidite monomer used for solid-phase oligonucleotide synthesis[1][2]

Storage Buffer: 20mM Tris-HCl

} DMT-2'-O-TBDMS-C(Ac)-CE-Phosphoramidite is a protected ribonucleoside building block used for solid-phase RNA oligonucleotide synthesis

Gian Maria

"Design of antisense oligonucleotides stabilized by locked nucleic acids

Therapeutic Oligonucleotide Research

The enzyme can add a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap structure at the 5' end of the RNA