pH-sensitive IgG labeling reagents plus(Green) OneStep ELISA Kit with C-terminal His&Avi tag LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGDGGGSGGGSHHHHHHHHHHGLNDIFEAQKIEWHE

with C-terminal His&Avi tag LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGDGGGSGGGSHHHHHHHHHHGLNDIFEAQKIEWHE

1) Take out the UA-Glo Kinase Max Luminescent Detection Reagent and equilibrate to room temperature

There is no loss of signal intensity after 4 repeated freeze-thaw cycles

R-spondin-1 Accession Q2MKA7-1 Amino Acid Sequence Ser21-Ala146

pH-sensitive IgG labeling reagents plus(Green) OneStep ELISA Kit with C-terminal His&Avi tag LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGDGGGSGGGSHHHHHHHHHHGLNDIFEAQKIEWHEProduct Specification Molecular Weight The product has a MW of 21. 8 kDa Physical Appearance Lyophilized Powder Storage Buffer Lyophilized from sterile PBS, pH 7. 4. Normally 5 % 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. Reconstitution 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3 5min at room temperature to ensure the