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the protein of interest is often cleaved from MBP with a specific protease and can then be separated from MBP by affinity chromatography
Protein phosphatase 1 regulatory subunit 71 (PPP1R71)
In different tissues
Measure the light signal and establish the conversion curve
UniOne® TR-FRET Human IL10/IL10RA Binding Kit Size:500T the protein of interest isProduct Specification Host Human Stability & Storage 80 Background This kit utilizes HomogeneousTime Resolved Fluorescence (TR FRET) technology to measure the interactionbetween IL10A and IL10RA. This method enables simple and rapid high throughputscreening of inhibitors and antibody blockers. As illustrated, the interactionbetween IL10A and IL10RA is detected using an Eu labeled Anti Tag1 antibody(TR FRET donor) and an Acceptor labeled IL10A protein