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Determine the amount of reagent to be prepared according to experimental needs
Within the ECD
Substitute the sample OD450 value into the standard curve to calculate the sample concentration
(2004) Int
Human CILP ELISA Kit Recombinant Protein Determine the amount of reagentProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and